88

Bioinformatics of the Brain

TABLE 3.1

Scaffold-free approaches of GBM models.

Technique

Cell source/cell line

Ref.

Hanging drop

DBTRG, U251

[166]

Inverted hanging drop chips

LN229,

patient-derived

mouse

xenograft cells

[167]

Methylcellulose modified hang-

ing drops

Patient derived GBM cells,

NCH82, HUVEC combinations

[162]

Medium in oil

UVW

[168]

Magnetic levitation

LN229, U251 (combined with)

human astrocytes

[152]

NASA HARV bioreactor

GBM patient derived cells

[169]

Uncoated or agarose coated well

plate

CSCs and umbilical cord MSCs

[146]

Agar coated flask

GBM patient derived cells, pri-

mary PDX-derived cells

[170]

Encapsulation in matrigel drops

with shaking culture in growth

factor (EGF, FGF-2) enriched,

serum-free culture medium

GBM patient derived cells

[155]

Round bottom ultra-low attach-

ment microplate (liquid overlay

technique)

U87, C8D1A astrocytes and HU-

VEC combination

[148]

Processing human GBM tissue

followed by culture in ultra low

attachment plates with shaking

in serum-free culture medium

Human GBM tissue

[156]

Processing into pieces and cul-

ture in nontreated, flat bottom

well plate on orbital shaker

Human GBM tissue

[157]

Growth factor (EGF, FGF-2)

enriched, serum-free culture

medium and ultra-low

attachment plates

U87, U251, A172, SF767,

primary GBM cells

[171]

3.4

Applications of GBM Models in Basic and Clinical

Research

As described in previous sections, scaffold-based, scaffold-free and hybrid

models of GBM have been developed with diverse techniques. This section